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horseradish peroxidase hrp substrate  (Vector Laboratories)


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    Structured Review

    Vector Laboratories horseradish peroxidase hrp substrate
    Horseradish Peroxidase Hrp Substrate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/SK-4705/ImmPACT+SG+Peroxidase+(HRP)+Substrate/bio_rxiv__64898__2026__03__06__710208-243-6-10
    Average 93 stars, based on 52 article reviews
    horseradish peroxidase hrp substrate - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Staining:

    Article Title: Discrete genetic effects of VHL and PBRM1 inactivation co-operate to disrupt epithelial homeostasis and promote ccRCC
    Article Snippet: .. Here, BrdU signal was visualized with the ImmPACT SG (grey) substrate (Vector SK4705), which contrasted with the DAB (brown) stain used to detect tdTomato on the same sections. ..

    Article Title: Hippocampal perineuronal net degradation identifies prefrontal and striatal circuits involved in schizophrenia-like changes in marmosets.
    Article Snippet: .. For DAB staining, PNNs were stained using biotinylated lectin from W. floribunda incubated for 24 hours at 4°C, followed by Vectastain Elite ABC Kit (Peroxidase PK- 6100, VectorLabs, US) for 1 hour at room temperature, and lastly reacted with SIGMAFAST DAB (brown; D4393- 50SET; Sigma- Aldrich, UK) for 1 min. To costain the PV interneurons, slices were incubated overnight at room temperature with an anti- PV rabbit primary antibody, then a goatanti- rabbit secondary antibody (ab6720) for 2 hours at room temperature, and ExtrAvidin- Perioxidase as a tertiary antibody (E2886; Sigma- Aldrich, UK) for 1.5 hours at room temperature before reacting with ImmPACT SG (gray; SK- 4705, VectorLabs) for 45 s. Sections were then mounted, dehydrated, and coverslipped with DPX (06522; Sigma- Aldrich) before imaging using a Hamamatsu Nanozoomer. .. To quantify the effectiveness of the chondroitinase enzyme at degrading PNNs in the marmoset, further analysis was conducted in MATLAB using the Image Processing Toolbox to identify and count PNNS from the brown DAB–stained pixels.

    Immunohistochemistry:

    Article Title: SMAD4 induces opposite effects on metastatic growth from pancreatic tumors depending on the organ of residence
    Article Snippet: Primary antibodies were to SMAD4 (clone EP618Y, Millipore, 04-1033; 1:200, IHC), pSMAD2 (clone 138D4, Cell Signaling, 3108; 1:100, IHC), p57 (Atlas Antibodies, HPA002924; 1:500, IHC), KLF4 (Abcepta, AM2725A; 1:100, IHC), KLF5 (Abcam, ab137676; 1:500, IHC), KLF6 (Abcam, ab241385; 1:1,000, IHC), RUNX1 (clone D4A6, Cell Signaling, 8529; 1:500, IHC), RUNX2 (clone D1L7F, Cell Signaling, 12556; 1:500, IHC), RUNX3 (clone 2B3, Life Technologies, MA5-17169; 1:500, IHC), mKate2 (Evrogen, AB233; 1:1,000, IF), Ki67 (clone B56, BD Pharmingen, 550609; 1:200, IF) and α-SMA (clone 1A4, Sigma, A2547; 1:1,000, IF). .. For IHC, horseradish peroxidase (HRP)-conjugated secondary antibodies (ImmPRESS kits, Vector Laboratories, MP7401 and #MP2400) were applied for 30–60 min at room temperature, followed by incubation with DAB substrate (ImmPACT kit, Vector Laboratories, SK-4105). .. Tissues were then stained with hematoxylin, dehydrated and mounted using Permount (Fisher Scientific, SP15-100).

    Article Title: SMAD4 induces opposite effects on metastatic growth from pancreatic tumors depending on the organ of residence.
    Article Snippet: Primary antibodies were to SMAD4 (clone EP618Y, Millipore, 04-1033; 1:200, IHC), pSMAD2 (clone 138D4, Cell Signaling, 3108; 1:100, IHC), p57 (Atlas Antibodies, HPA002924; 1:500, IHC), KLF4 (Abcepta, AM2725A; 1:100, IHC), KLF5 (Abcam, ab137676; 1:500, IHC), KLF6 (Abcam, ab241385; 1:1,000, IHC), RUNX1 (clone D4A6, Cell Signaling, 8529; 1:500, IHC), RUNX2 (clone D1L7F, Cell Signaling, 12556; 1:500, IHC), RUNX3 (clone 2B3, Life Technologies, MA5-17169; 1:500, IHC), mKate2 (Evrogen, AB233; 1:1,000, IF), Ki67 (clone B56, BD Pharmingen, 550609; 1:200, IF) and α-SMA (clone 1A4, Sigma, A2547; 1:1,000, IF). .. For IHC, horseradish peroxidase (HRP)-conjugated secondary antibodies (ImmPRESS kits, Vector Laboratories, MP7401 and #MP2400) were applied for 30–60 min at room temperature, followed by incubation with DAB substrate (ImmPACT kit, Vector Laboratories, SK-4105). .. Tissues were then stained with hematoxylin, dehydrated and mounted using Permount (Fisher Scientific, SP15-100).

    Incubation:

    Article Title: SMAD4 induces opposite effects on metastatic growth from pancreatic tumors depending on the organ of residence
    Article Snippet: Primary antibodies were to SMAD4 (clone EP618Y, Millipore, 04-1033; 1:200, IHC), pSMAD2 (clone 138D4, Cell Signaling, 3108; 1:100, IHC), p57 (Atlas Antibodies, HPA002924; 1:500, IHC), KLF4 (Abcepta, AM2725A; 1:100, IHC), KLF5 (Abcam, ab137676; 1:500, IHC), KLF6 (Abcam, ab241385; 1:1,000, IHC), RUNX1 (clone D4A6, Cell Signaling, 8529; 1:500, IHC), RUNX2 (clone D1L7F, Cell Signaling, 12556; 1:500, IHC), RUNX3 (clone 2B3, Life Technologies, MA5-17169; 1:500, IHC), mKate2 (Evrogen, AB233; 1:1,000, IF), Ki67 (clone B56, BD Pharmingen, 550609; 1:200, IF) and α-SMA (clone 1A4, Sigma, A2547; 1:1,000, IF). .. For IHC, horseradish peroxidase (HRP)-conjugated secondary antibodies (ImmPRESS kits, Vector Laboratories, MP7401 and #MP2400) were applied for 30–60 min at room temperature, followed by incubation with DAB substrate (ImmPACT kit, Vector Laboratories, SK-4105). .. Tissues were then stained with hematoxylin, dehydrated and mounted using Permount (Fisher Scientific, SP15-100).

    Article Title: SMAD4 induces opposite effects on metastatic growth from pancreatic tumors depending on the organ of residence.
    Article Snippet: Primary antibodies were to SMAD4 (clone EP618Y, Millipore, 04-1033; 1:200, IHC), pSMAD2 (clone 138D4, Cell Signaling, 3108; 1:100, IHC), p57 (Atlas Antibodies, HPA002924; 1:500, IHC), KLF4 (Abcepta, AM2725A; 1:100, IHC), KLF5 (Abcam, ab137676; 1:500, IHC), KLF6 (Abcam, ab241385; 1:1,000, IHC), RUNX1 (clone D4A6, Cell Signaling, 8529; 1:500, IHC), RUNX2 (clone D1L7F, Cell Signaling, 12556; 1:500, IHC), RUNX3 (clone 2B3, Life Technologies, MA5-17169; 1:500, IHC), mKate2 (Evrogen, AB233; 1:1,000, IF), Ki67 (clone B56, BD Pharmingen, 550609; 1:200, IF) and α-SMA (clone 1A4, Sigma, A2547; 1:1,000, IF). .. For IHC, horseradish peroxidase (HRP)-conjugated secondary antibodies (ImmPRESS kits, Vector Laboratories, MP7401 and #MP2400) were applied for 30–60 min at room temperature, followed by incubation with DAB substrate (ImmPACT kit, Vector Laboratories, SK-4105). .. Tissues were then stained with hematoxylin, dehydrated and mounted using Permount (Fisher Scientific, SP15-100).

    Article Title: Hippocampal perineuronal net degradation identifies prefrontal and striatal circuits involved in schizophrenia-like changes in marmosets.
    Article Snippet: .. For DAB staining, PNNs were stained using biotinylated lectin from W. floribunda incubated for 24 hours at 4°C, followed by Vectastain Elite ABC Kit (Peroxidase PK- 6100, VectorLabs, US) for 1 hour at room temperature, and lastly reacted with SIGMAFAST DAB (brown; D4393- 50SET; Sigma- Aldrich, UK) for 1 min. To costain the PV interneurons, slices were incubated overnight at room temperature with an anti- PV rabbit primary antibody, then a goatanti- rabbit secondary antibody (ab6720) for 2 hours at room temperature, and ExtrAvidin- Perioxidase as a tertiary antibody (E2886; Sigma- Aldrich, UK) for 1.5 hours at room temperature before reacting with ImmPACT SG (gray; SK- 4705, VectorLabs) for 45 s. Sections were then mounted, dehydrated, and coverslipped with DPX (06522; Sigma- Aldrich) before imaging using a Hamamatsu Nanozoomer. .. To quantify the effectiveness of the chondroitinase enzyme at degrading PNNs in the marmoset, further analysis was conducted in MATLAB using the Image Processing Toolbox to identify and count PNNS from the brown DAB–stained pixels.

    other:

    Article Title: Fasting primes small intestinal regeneration after damage via a microbiome–metabolite-chromatin axis
    Article Snippet: After washing with PBS, endogenous peroxidase activity was blocked with Dual Endogenous Enzyme Block (Dako # S2003) for 10 minutes at room temperature.

    Imaging:

    Article Title: Hippocampal perineuronal net degradation identifies prefrontal and striatal circuits involved in schizophrenia-like changes in marmosets.
    Article Snippet: .. For DAB staining, PNNs were stained using biotinylated lectin from W. floribunda incubated for 24 hours at 4°C, followed by Vectastain Elite ABC Kit (Peroxidase PK- 6100, VectorLabs, US) for 1 hour at room temperature, and lastly reacted with SIGMAFAST DAB (brown; D4393- 50SET; Sigma- Aldrich, UK) for 1 min. To costain the PV interneurons, slices were incubated overnight at room temperature with an anti- PV rabbit primary antibody, then a goatanti- rabbit secondary antibody (ab6720) for 2 hours at room temperature, and ExtrAvidin- Perioxidase as a tertiary antibody (E2886; Sigma- Aldrich, UK) for 1.5 hours at room temperature before reacting with ImmPACT SG (gray; SK- 4705, VectorLabs) for 45 s. Sections were then mounted, dehydrated, and coverslipped with DPX (06522; Sigma- Aldrich) before imaging using a Hamamatsu Nanozoomer. .. To quantify the effectiveness of the chondroitinase enzyme at degrading PNNs in the marmoset, further analysis was conducted in MATLAB using the Image Processing Toolbox to identify and count PNNS from the brown DAB–stained pixels.



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